Protocols from the Thomas Lab Preparation of the Filamentous Bacteriophage Ff Preparation of the Filamentous Bacteriophage Pf1 Preparation of Isotopically-Labeled Filamentous Virus Preparation of the Filamentous Bacteriophage Pf3 Purification of pUC19 (modified Qiagen protocol) Purification of HUBst Protein Protocol for P22 His6-Tagged Portal Protein Expression Protocol for P22 Phage Preparation Protocol for making mutants (arginine auxotrophs) of Pseudomonas aeruginosa strain K Preparation of Linear 217-bp DNA Circularization of 217-bp Linear DNA Fragments IHF Purification Protocol Hydrolysis of dsDNA for extinction coefficient calculation
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