Flow Cytometry
Director, Tara Allen

   303 BSB
    235-2568

Capabilities:

  • Determination of cell size (Forward Scatter – FSC) and cell complexity (Side Scatter – SSC)
  • Measurement of fluorescent intensity (FL1, FL2, FL3) to assess cell viability, apoptosis, total DNA content, protein expression, cell surface or intracellular antigen presence, intracellular calcium, etc.
  • Cell identification and sorting/isolating a subpopulation of cells from the sample

Equipment:

  • Becton Dickinson FACSCalibur
  • FlowJo Software for analysis of cytometric data

Resources: