- Determination of cell size (Forward Scatter – FSC) and cell complexity (Side Scatter – SSC)
- Measurement of fluorescent intensity (FL1, FL2, FL3) to assess cell viability, apoptosis, total DNA content, protein expression, cell surface or intracellular antigen presence, intracellular calcium, etc.
- Cell identification and sorting/isolating a subpopulation of cells from the sample
- Becton Dickinson FACSCalibur
- FlowJo Software for analysis of cytometric data