Published Method Papers

1. Waterborg, J.H. Acid-Urea Polyacrylamide Gels for Basic Proteins in "The Protein Protocols Handbook", Ed. J.M. Walker, pp. 83-90. Humana Press, NJ, 1996.
2. Waterborg, J.H. Acid-Urea-Triton Polyacrylamide Gels for Histones in "The Protein Protocols Handbook", Ed. J.M. Walker, pp. 91-100. Humana Press, NJ, 1996.
3. Kapros, T. and Waterborg, J.H. Rapid Extraction of Multiple RNA Samples from Plant Suspension Cells Focus 17: 20-21, 1995.
4. Kapros, T., Robertson, A.J. and Waterborg, J.H. A Simple Method to make Better Probes from Short DNA Fragments Mol. Biotechnol. 2: 95-98, 1994. (Abstract)
5. Waterborg, J.H. and Matthews, H.R. The Lowry Method for Protein Quantitation in "Methods in Molecular Biology" Vol. 32 Basic Protein and Peptide Protocols, Ed. J.M. Walker, Humana Press, NJ, pp 1-4, 1994.
6. Waterborg, J.H. and Matthews, H.R. Fluorography of Polyacrylamide Gels Containing Tritium in "Methods in Molecular Biology" Vol. 32 Basic Protein and Peptide Protocols, Ed. J.M. Walker, Humana Press, NJ, pp 163-167, 1994.
7. Waterborg, J.H. and Matthews, H.R., "The Electrophoretic Elution of Proteins From Polyacrylamide Gels," in Methods in Molecular Biology. Vol. 32 "Basic Protein and Peptide Protocols", Ed. J.M. Walker, Humana Press, NJ, pp 169-175, 1994.
8. Waterborg, J.H. and Robertson, A.J. Efficient Large-scale Purification of Restriction Fragments by Solute-displacement Ion-exchange HPLC Nucleic Acids Res. 21: 2913-2915, 1993. (Abstract)
9. Waterborg, J.H. Involvement of Cysteine Residues in the Electrophoretic Mobility of Histone H3 in Acid-Urea-Triton Gels Electrophoresis 11: 638-641, 1990. (Abstract)
10. Waterborg, J.H., Pitter, R.L. and Harrington, R.E. A Low-Cost, High-Capacity Data Acquisition System for Apple II Microcomputers Access 8: 14-25, 1989.
11. Waterborg, J.H. and Harrington, R.E. Western Blotting of Histones to S&S NC Nitrocellulose Membranes Sequences 28: 4-5, 1989.
12. Waterborg, J.H. and Harrington, R.E. Western Blotting of Histones From Acid-Urea-Triton and SDS Polyacrylamide Gels Anal. Biochem. 162: 430-434, 1987.
13. Waterborg, J.H. and Harrington, R.E. A Standard Multidimensional, Easy-Access Data File Structure for Apple II Computers Computer Methods and Programs in Biomedicine 23: 255-260, 1986.
14. Waterborg, J.H. and Matthews, H.R. The Lowry Method for Protein Quantitation in "Methods in Molecular Biology" Vol. 1 Proteins, Ed. J.M. Walker, Humana Press, NJ, pp 1-3, 1984.
15. Waterborg, J.H. and Matthews, H.R. Fluorography of Polyacrylamide Gels Containing Tritium in "Methods in Molecular Biology" Vol. 1 Proteins, Ed. J.M. Walker, Humana Press, NJ, pp 147-152, 1984.
16. Waterborg, J.H. and Matthews, H.R. Recovery of Proteins From Dried Polyacrylamide Gels After Fluorography in "Methods in Molecular Biology" Vol. 1 Proteins, Ed. J.M. Walker, Humana Press, NJ, pp 153-155, 1984.
17. Waterborg, J.H. and Matthews, H.R. The Electrophoretic Elution of Proteins From Polyacrylamide Gels in "Methods in Molecular Biology" Vol. 1 Proteins, Ed. J.M. Walker, Humana Press, NJ, pp 157-164, 1984.
18. Waterborg, J.H. and Matthews, H.R. The Burton Assay for DNA in "Methods in Molecular Biology" Vol. 2 Nucleic Acids Ed. J.M. Walker, Humana Press, NJ, pp 1-3, 1984.

Abstracts

A Simple Method to make Better Probes from Short DNA Fragments
Kapros, T; Robertson, AJ; Waterborg, JH
Mol. Biotechnol. 1994 Aug; 2(1): 95-98
A detailed method is presented for the creation of head-to-tail multimers of short blunt restriction fragments, ligated into a plasmid vector in a single-tube reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments.

Efficient Large-scale Purification of Restriction Fragments by Solute-displacement Ion-exchange HPLC
Waterborg, JH; Robertson, AJ
Nucleic Acids Res. 1993 Jun 25; 21(12): 2913-2915
Extreme overloading of HPLC columns with sample can create a condition of binding site saturation causing competition and displacement among solutes during column elution. This has been termed solute-displacement chromatography (SD-HPLC). We present an example of this phenomenon for the preparative fractionation and purification of restriction fragments of almost identical size (1337 and 1388 bp) which cannot be resolved by agarose gel electrophoresis. Standard analytical ion-exchange HPLC chromatography failed to separate these fragments from each other and from an unexpectedly early eluting pUC-derived vector fragment of 2.7 kbp. We demonstrate that by intentional overloading of the small (4.6 x 35 mm) non-porous TSK-DEAE HPLC column, hundreds of micrograms of DNA restriction fragments could be resolved and purified in a single HPLC run of less than 30 minutes.

Involvement of Cysteine Residues in the Electrophoretic Mobility of Histone H3 in Acid-urea-Triton Gels
Waterborg, JH
Electrophoresis 1990 Aug; 11(8): 638-641
Carbamylation of cysteines 96 and 110 in histone H3 increases the electrophoretic mobility of this histone in acetic acid-urea-Triton X-100 polyacrylamide gels but has no effect in gels lacking Triton. Residue 96 appears to be a major determinant in the affinity of histone H3 for the nonionic detergent Triton. Carbamylation and carboxymethylation of cysteine 96 caused a major loss of the gel retardation caused by Triton. Carbamylation of cysteine 110 did not affect Triton binding but prevented ionization of the thiol side-chain moiety in the acetic acid-urea-Triton X-100 gel.