Facilities

Modern biological research is a complex effort, aided at SBS by the availability of state-of-the-art facilities and a highly-skilled technical staff for the analysis and interpretation of experimental results. We have a large number of central facilities equipped for modern research and located in the Biological Sciences/Spencer Chemistry building complex.

Genomics
 Director, Leonard Dobens  306B SCB  (816) 235-6272  DobensL@umkc.edu

Capabilities:

  • DNA sequencing of dsDNA, ssDNA and purified PCR products
  • Real-time PCR

Equipment:

  • ABI 7500 Real-time PCR
  • ABI 3100 Genetic Analyzer from Applied Biosystems, 50 cm x 16 capillary array
  • Typhoon 9400 Variable Mode Imager for phosphorimaging, chemiluminescence and fluorescence
  • Storm 840 Imager by GE Healthcare for phosphorimaging and fluorescence
  • Kodak Gel Logic 200 Imaging System
Proteomics and Mass Spectrometry Facility
Director, Andrew Keightley 405C SCB (816) 235-2565 KeightleyJ@umkc.edu

Capabilities and services:

  • In-gel trypsin digestion, and FASP (filter aided sample preparation-samples in solution)
  • Protein identification by LC-tandem MS
  • Whole peptidome analysis using multi-dimensional liquid chromatography (mudpit)
  • Tandem Mass Tag-based quantitation/differential expression analysis
  • Database searches using Proteome Discoverer and Mascot

Equipment:

  • Q Exactive Plus High Resolution Mass Spectrometer (HRMS)
  • Thermo Finnigan LTQ linear ion trap
  • Eksigent binary nano-LC pump system (1D nanoLC)
  • Eksigent quaternary nano-LC pump system (2D nanoLC)
  • CTC PAL robot for fraction collection (1st dimension for mudpit), 96 well format
  • Dell Xeon 3.7 processor, 36Gb memory, 400Gb SSD boot, spinning 2 x 4Tb archives

Sample Submission Guide, Sample Form and Fees

High Field NMR Laboratory
Acting Director, Theodore White 198 BSB (816) 235-2538 whitetc@umkc.edu

Capabilities:

  • Data collection for academic researchers
  • NMR data analysis training by arrangement
  • NMR data collection training for frequent users

Equipment:

  • Oxford AS 600/51 14.1 Tesla actively shielded magnet
  • {HCN} cryo and {HCX} RT probes
  • Four full channels with observe, pulse and decouple functions
  • Two Sun Blade 1000 and three Linux-based computers; an additional Linux computer is available within SBS
X-ray Crystallography Laboratory
Director, Marilyn D. Yoder 526 SCB (816) 235-1986 YoderM@umkc.edu

Capabilities:

  • Crystallization screens
  • X-ray data collection

Equipment:

  • UMKC is part of the Southeast Regional Collaborative Access Team operating two beamlines at the Advanced Proton Source at Argonne National Laboratories
Electron Microscopy Laboratory
Director, Edward Gogol 192 BSB (816) 235-2584 GogolE@umkc.edu

Capabilities:

  • Visualization of macromolecules, viruses and bacteria by negative staining

Equipment:

  • JEOL 1200-EX TEM equipped with goniometric specimen stage
Flow Cytometry
Director, Tara Allen 275F BSB (816) 235-2568 allent@umkc.edu

Capabilities:

  • Determination of cell size (Forward Scatter – FSC) and cell complexity (Side Scatter – SSC)
  • Measurement of fluorescent intensity (FL1, FL2, FL3) to assess cell viability, apoptosis, total DNA content, protein expression, cell surface or intracellular antigen presence, intracellular calcium, etc.
  • Cell identification and sorting/isolating a subpopulation of cells from the sample

Equipment

  • Becton Dickinson FACSCalibur
  • FlowJo Software for analysis of cytometric data

Resources

Protein Interaction and Dynamics
Director, John Laity 114 SCB (816) 235-5345 LaityJ@umkc.edu

Capabilities:

  • Circular-dichroism
  • Surface plasmon resonance
  • Analytical ultracentrifugation
  • Microcalorimetry

Equipment:

  • Jasco model J-720 spectropolarimeter – circular dichroism (CD)
  • Beckman X-LA analytical ultracentrifuge
  • PTI photon counting fluorometer with fluorescence lifetime and depolarization capabilities
  • FLUOstar Plate reader
  • MicroCal VP-ITC
  • Dynapro dynamic light scattering instrument, with temperature control
  • Biacore X
Fluorescence Microscopy
Director, Jeffrey Price 406B SCB (816) 235-2572 PriceJL@umkc.edu

Capabilities:

  • Total internal reflectance fluorescence (TIRF) microscopy
  • Optical sectioning by confocal laser scanning (LSM) and structured illumination (SIM) microscopy
  • 3D image reconstruction by deconvolution optical microscopy

Equipment:

  • Nikon TIRF inverted microscope
  • Olympus DeltaVision inverted microscope
  • Nikon OptiGRID SIM upright microscope
  • Zeiss LSM-410 inverted microscope
  • Zeiss LSM-5 Pascal upright microscope
  • Olympus LSM upright microscope